FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42220221
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG572420.13557958401023057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG12450.017.3448496
AGACCGC46700.017.3423634
GCCGGCT193250.016.6174741
AACGCTA195350.016.44338223
ACGCTAC197600.016.290324
CCGTACG76700.016.014744
CGTACGC78550.015.8667795
CGCTACC202350.015.81917125
TTCACGC215650.015.5211787
CCGGCTT222400.015.4162232
AAAACGC210350.015.30296221
GCCTACG20350.015.25412810
TCGGCGC84200.015.01647445
CCGGTAT32050.014.9558841
CAGACCG55850.014.8636623
GAAAACG215850.014.82966120
TTCGGCG84800.014.80469844
TCACGCT229050.014.6623058
CTCGGTT81950.014.609091
TACGCCA88150.014.5963727
Produced by FastQC (version 0.11.5)