FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42220221
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA510890.12100599852378792No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG429360.10169534640758987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG153400.014.15321838
GGCATAG151150.014.08117235
GGGAGGT242350.013.8375443
AGCTTAG161950.013.10064545
GGAGGTC252000.013.0575784
GAGCTTA164600.012.94454344
CACCGAA235800.012.776113545
TGGCATA168050.012.73203534
CGTGGCG88350.012.3298621
CATAGGG174550.012.14163237
TTCTATA280150.011.5045392
GCGTAGG89800.011.19910636
CGTAGGG89950.011.15526137
GCATAGG193050.011.07148836
GGCGTAG90850.011.06976535
GCACCGA242100.011.05907544
TGCCCGT124150.011.01852544
TTTCTAT303350.010.9512021
GTTGTCG16000.010.8278326
GTGGCGA100450.010.7997552
Produced by FastQC (version 0.11.5)