FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47244048
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA595630.12607514072460513No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG549420.1162940144333102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT169750.018.3063431
TCGTTAC28900.018.06072644
TCGGGGC122550.017.40363944
GACCGCG26900.017.3937645
TTCGGGG123800.017.3572443
AACGCTA169500.017.22601523
ACGCTAC170600.017.08857224
ATTCGGG131100.016.71914342
CGCTACC175150.016.55511525
CCGGCTT204250.016.1460042
TTCACGC189050.016.1228717
TCACGCT187650.016.0993088
ACCGCGT29550.015.9861176
AATTCGG136300.015.86740541
AAAACGC186500.015.72818421
TAATTCG140900.015.46339540
GAAAACG190100.015.35933220
ACGCTCA196450.015.32090210
AAACGCT195550.015.0808122
TAATCTA150500.015.0213799
Produced by FastQC (version 0.11.5)