FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44047440
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA5044871.1453264934352598No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC2218880.5037477773963709No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA1911710.43401160203634986No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC1293550.2936720045478239No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC1011820.22971142023236762No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCC872710.19812956212665253No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC713590.1620048747441395No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTA591480.13428249178612878No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCC573680.1302413942785324No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA543140.12330796068965642No Hit
CCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG542500.12316266280174284No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCT541890.12302417575232522No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACG529440.12019767777650642No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGT529220.12014773162753611No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACT522620.11864934715842737No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC519350.11790696576236892No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT511280.11607485020695867No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTC510190.11582738974160586No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGG492530.1118180761469906No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG487630.11070563919265228No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC486410.11042866509381702No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC479960.10896433481718802No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA467400.10611286376688406No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG454090.1030911217541814No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG448810.10189241417889439No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA440930.10010343393395847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGAC763800.032.9045141
ACAGACT790400.031.6847252
AGACTCA1048000.024.1810324
CAGACTC1043000.024.1631453
GACTCAG1091550.023.1725275
ACTCAGA1093950.023.1130896
TCAGAGA1352150.018.5887268
CTCAGAG1365750.018.441517
CAGAGAG1467200.017.0491649
AGAGAGA1524850.016.46655710
CACAACT165950.016.3281541
AGAACCC1533200.016.19572314
AACCCAC1536000.016.14836516
AGAGAAC1546600.016.08951212
ACCCACC1561650.015.95949117
GAACCCA1574700.015.75460915
GAGAGAA1600950.015.6782111
GAGAACC1589650.015.67218513
CAACTGT255550.014.3932551
CCACCAT1776950.014.05111919
Produced by FastQC (version 0.11.5)