FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44648697
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA82250.024.23049223
ACGCTAC85750.023.45134524
CGCTACC87000.022.64918725
AAAACGC95700.020.77804821
GCCGGCT192350.020.2670351
CGTGGTT187450.019.42453441
GAAAACG105450.019.07020420
GTTTTCG196150.018.66128245
TTCACGC205700.018.5243327
TCACGCT208300.018.3687028
CCGGCTT214050.018.3207552
AAACGCT110050.018.19132622
TCGTGGT203050.017.96643440
CACGCTC233450.016.4765269
ACGCTCA235000.016.0998410
CTACCTC232850.016.09537527
CCTCGTG236400.015.72999738
CCCGTAT30300.015.5521741
TTCCTCG244100.015.45511636
CGGCTTC267400.015.4543623
Produced by FastQC (version 0.11.5)