FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45599585
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTTG710210.15574922447210868No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG566860.12431253486188526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA232400.027.8752623
ACGCTAC235050.027.58012824
CGCTACC238650.027.06094225
GCCGGCT246950.026.694841
AAAACGC247300.026.27761721
GAAAACG256100.025.29568320
AAACGCT259950.025.03347822
CCGGCTT283800.023.8302562
TTCACGC284900.023.3700397
CGTGGTT280500.023.00941541
TCGTGGT284450.022.66767740
TCACGCT294600.022.661648
ACGCTCA299050.022.23416310
CACGCTC319650.020.9490499
CCTCGTG324500.020.1860138
CGGCTTC351650.019.7352813
CTCGTGG337600.019.42824639
TTCCTCG340100.019.4189536
TACCTCT347200.018.79359628
AGAAAAC355650.018.74646619
Produced by FastQC (version 0.11.5)