FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43876459
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG543930.12396852717763757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG27100.022.09606732
AACGCTA188250.017.67162123
ACGCTAC191400.017.42787424
GCCGGCT200800.017.175921
CGCTACC194400.017.05490725
TTCGACG14350.016.7722136
TCGTATG37500.016.13783845
TCACGCT217000.016.0973348
AAAACGC207650.016.08559821
CCCGTAT30250.016.032061
GCCTACG29150.015.81821610
CGTCCGC38300.015.81090333
GAAAACG214750.015.51188520
CCGGTAT58800.015.4214431
TTCACGC235350.015.1671827
ACGCTCA234000.015.04321610
AAACGCT223100.014.95149922
GTCGCTA28400.014.89215127
GTTCCTA74700.014.84461815
ATGCGCG24700.014.75638744
Produced by FastQC (version 0.11.5)