FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45136303
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG3218110.7129759829908976No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG866920.19206712610024795No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG514990.11409662860513853No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC462340.10243196036680276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT652250.024.4168261
CCGGCTT741650.023.029212
AACGCTA769400.021.87346823
ACGCTAC769950.021.85200124
CGCTACC778700.021.52901525
AAAACGC785550.021.466321
TTCACGC797150.021.3510157
GAAAACG788300.021.334320
CGGCTTC829050.021.2547253
TCACGCT809750.021.1021148
AAACGCT801400.021.03935222
ACGCTCA834500.020.64067310
CACGCTC847900.020.1234789
GTTTTCG843200.019.57807445
CGTGGTT855800.019.4565641
TCGTGGT862400.019.32079540
GGCTTCA940100.019.1268184
ACCGCGT70950.019.084426
CGCTCAG917050.018.87586611
AGACCGC71500.018.8754734
Produced by FastQC (version 0.11.5)