FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41840219
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG628100.15011871711283348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT191600.019.086421
CCGGCTT214450.017.4035132
TTCACGC212450.017.1735827
AACGCTA208700.016.7495523
ACGCTAC210550.016.66648124
CGCTACC213450.016.32439425
TCACGCT228500.015.8393988
AAAACGC225000.015.59619721
AGACCGC37250.015.4001444
ACGCTCA236850.015.38547910
AAACGCT233100.015.08319422
CGTGGTT233500.015.06443141
GAAAACG234550.014.87486320
CGTTACG14150.014.78438121
CGGCTTC270000.014.7558993
CACGCTC249300.014.517869
GTTTTCG242650.014.41765845
CTCCGAT39350.014.411941
ACCGCGT41800.013.9376556
CCGGTAT31200.013.8488361
Produced by FastQC (version 0.11.5)