FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42613327
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG613450.14395731175836143No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG460480.10806009115411241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT155850.022.0610031
TTCACGC271750.021.59567
TCACGCT278100.021.045888
ACGCTCA289550.020.29909110
CCGGCTT182950.019.7500022
CACGCTC304800.019.1949129
ACGCTAC305200.018.64649824
AACGCTA305650.018.60432623
CGCTACC310300.018.25338425
GACCGCG37400.017.984155
ACCGCGT38550.017.797786
AAAACGC320950.017.72444721
CGCTCAG339800.017.37667711
GAAAACG330050.017.27666920
AGACCGC39050.017.224524
AAACGCT336100.016.93888722
CGGCTTC236900.016.2758143
CAGACCG44500.015.367763
CGTGGTT381950.014.84189541
CCCGTAT39500.014.8401571
Produced by FastQC (version 0.11.5)