FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44533106
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG700300.15725379675965112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC47050.019.4129414
GCCGGCT212700.018.462891
GACCGCG50050.018.2045235
ACCGCGT53300.017.5563036
AACGCTA219200.017.2914623
ACGCTAC220700.017.21470524
CCGGCTT236100.017.1516282
TCACGCT233400.016.9428468
TTCACGC233700.016.940297
CGCTACC225500.016.7486925
CAGACCG56100.016.5619753
ACGCTCA245650.016.18039710
AAAACGC236350.015.95108421
GAAAACG239900.015.63065420
CACGCTC264250.015.0329469
CCCGTAT31800.015.0030831
AAACGCT252600.014.93387522
CGGCTTC298550.014.64886953
CCGCGTT65050.014.5587
CGTACGC67550.013.6881535
Produced by FastQC (version 0.11.5)