FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46383012
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1521020.32792609501081993No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA515540.111148452368725No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG472780.10192955990007721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT335000.023.421931
CCGGCTT394200.021.1030852
ACCGCGT26750.020.098226
TTCACGC418750.019.2743267
GACCGCG28900.018.9144955
ACGCTCA428900.018.88115510
TCACGCT434400.018.5851178
CGGCTTC476400.018.0096233
CACGCTC464750.017.4246969
CGCTCAG479000.016.9063211
AACGCTA473400.016.69764123
ACGCTAC478150.016.53176724
CGCTACC483200.016.29899625
AAAACGC492100.016.07226621
CCCGTAT38450.015.8594641
GAAAACG499950.015.77041220
AAACGCT503950.015.68094822
CCGCGTT45550.015.3634081
GGCTTCA578250.015.3510164
AGACCGC34700.015.2347054
Produced by FastQC (version 0.11.5)