FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41009724
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA470610.11475571013352832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT26000.018.68211711
TCGTGTA31300.015.80638314
GTCGCCG33850.014.94172344
GTCGTGT34550.014.25430313
AGCGTCG35750.014.02719410
CGTCGTG34950.014.02668312
CACCGAA220100.012.81664745
GCACCGA233300.012.11716144
GGTCGCC39600.012.08793143
GACGCAA49700.012.08471944
ATAGGGG106800.012.04378638
GTTGTCG22800.011.93404626
ACGCAAG50500.011.80713645
GGCATAG108150.011.74817135
GGTCACT155850.011.7161757
CGTGGCG65750.011.525091
GTCACTC163500.011.4843238
GGGAGGT172850.011.3573583
TCTATAG262000.011.080453
TGCCCGT116600.010.9604744
Produced by FastQC (version 0.11.5)