FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40561087
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA111500.016.8659323
ACGCTAC113700.016.57915724
TTCACGC142350.016.4183087
GCCGGCT159500.016.2397481
CCGGCTT155800.016.0567822
CGCTACC117650.015.88890325
GAAAACG82400.015.3695520
TCACGCT155550.015.2998468
AAAACGC125250.015.12217721
GTTAGTT96000.014.8350544
ACGCTCA164200.014.5897510
CGCGTGT13400.014.2750891
CGCGTAA9850.014.1651171
AAACGCT139500.013.57743822
CACGCTC176250.013.464649
GTTTTCG166200.013.18482345
CGGCTTC197900.013.0501073
CGTGGTT170300.012.97663441
GGTTAGT108950.012.92875243
GCCCGCT80700.012.8555921
Produced by FastQC (version 0.11.5)