FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40561087
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAAC168900.013.94634337
CGTGGCG93950.013.7272751
ACTAACC174100.013.4526138
GGGAGGT219000.013.4016043
GGAGGTC226000.012.7076594
CTAACCA185050.012.64466839
ATAGGGG144450.012.60043638
TGCCCGT162450.012.451014544
GGCATAG143800.012.1878435
GTGGCGA108650.011.8078582
CGTAGGG80550.011.64699437
GCGTAGG82250.011.48845436
GGCGTAG82750.011.44622535
AGCTTAG161250.011.34366745
GCCCGTG181550.011.27736945
TGGCGAC116800.011.1572983
TGGCATA161250.011.10609934
CGACCCG116900.011.0869296
GAGCTTA164300.010.88665244
GGCGACC120250.010.7436074
Produced by FastQC (version 0.11.5)