FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44331441
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG796960.17977308700612732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC55100.020.0039424
GCCGGCT223350.019.182331
CCGGCTT251250.017.8897232
CAGACCG64200.017.518873
TCACGCT248650.017.3319458
TTCACGC252050.017.2320047
ACCGCGT67400.016.5530556
AACGCTA256250.016.31769223
ACGCTAC258550.016.20738624
CGCTACC264450.015.75228425
ACGCTCA280700.015.72961610
GACCGCG71450.015.583795
CACGCTC281400.015.2668529
AAAACGC276750.015.14954221
CGGCTTC312200.015.1306483
CCCGTAT26150.014.9257711
GAAAACG281600.014.8327120
CCGTACG77300.014.8118534
AAACGCT288400.014.6156322
TCGGCGC79350.014.37403445
Produced by FastQC (version 0.11.5)