FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44331441
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG854120.19266687044980108No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA552290.12458200941404093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT382850.017.2397253
GGAGGTC403200.016.28054
GGTCACT420300.015.1656857
GTCACTC424950.014.9360488
GGCATAG284000.014.81750135
ATAGGGG284150.014.77806838
ACTCTCC431100.014.70023811
AGCTTAG292400.014.43084245
GAGGTCA459800.014.315545
AGGTCAC464100.014.0547876
CACTCTC451500.014.05151310
TGGCATA304900.013.87580834
TGGGAGG327000.013.7280742
GAGCTTA308450.013.68735744
TCACTCT483000.013.3962369
GTTGTCG21300.013.09544926
CTGGGAG376250.012.9584851
TAGGGGA516300.012.34598139
TCTCCCC519150.012.22846413
GCTCTGT533700.012.16457822
Produced by FastQC (version 0.11.5)