FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44086614
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG595100.1349842834380522No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT468860.10634974144306025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18650.021.8358946
AGACCGC102000.021.462534
CAGACCG106250.020.8581393
GACCGCG104650.020.8115465
ACCGCGT106900.020.373656
TCGACGA23600.017.3512427
CCGCGTT126800.017.1939537
GCCGGCT202350.017.1333561
ACGCTAC205950.016.26702124
AACGCTA206000.016.26320323
CGCGTTC134800.016.206928
CCGGCTT228050.015.9436372
CGCTACC210300.015.877048525
TCACGCT222050.015.7561768
TTCACGC226700.015.6314847
CGGTATA33000.015.61406220
TCAGACC154700.015.1985472
GCCTACG32600.015.18370510
AAAACGC224350.015.01323621
CTCAGAC183950.014.8329581
Produced by FastQC (version 0.11.5)