FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44086614
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA628890.1426487414070856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC243350.013.694718
ATAGGGG160100.013.51206538
GGGAGGT268450.013.2517783
GGAGGTC267500.013.2316654
GGCATAG163950.013.09904935
ACTCTCC261900.012.94841411
GGTCACT256850.012.9048627
AGCTTAG175250.012.35883745
CGTAGGG101200.012.11039637
GCGTAGG101850.012.07728136
GGCGTAG103000.012.02990435
CACTCTC284500.011.666697510
CACCGAA288000.011.60609845
GAGCTTA187750.011.60514544
TCTATAG299150.011.4783933
GAGGTCA309800.011.4540515
TCACTCT296900.011.4367269
TGGCGTA110000.011.26436534
TTCTATA321000.011.1662152
TGGCATA192850.011.15938134
Produced by FastQC (version 0.11.5)