FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44305215
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA729290.16460590474507347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA311650.014.8061645
GCACCGA341500.013.59044744
ATAGGGG123650.012.67546938
GGCATAG121600.012.44555435
TAGCGTA5300.012.30413137
AGCTTAG124950.012.0748445
AGGGATC154700.011.9770282
GGGAGGT211900.011.6518223
TTTCTAT419550.011.581651
TTCTATA416800.011.4803742
GTTGTCG16000.011.38407426
GAGCTTA136550.011.24682144
CATAGGG136900.011.20236637
TCTATAG430050.010.9439143
TAGGGGA212700.010.62455439
TAGAGCA449950.010.61618840
TGGCATA144200.010.49500234
AGCACCG444250.010.46238143
GTAGAGC449750.010.42430339
CAGGGAT204600.010.3964461
Produced by FastQC (version 0.11.5)