FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46830811
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC35900.020.88033532
CGTCCCG43950.018.18233935
ATGCGCG26250.017.91522844
TGCGCGT26000.017.74010545
CCCGTAT31100.016.9295391
TCGTATG44800.016.9249645
GTCGCTA26700.016.599827
ACGCTAC142850.016.48757624
GCCGGCT154500.016.3546371
CCGGTAT60300.016.3062381
AACGCTA143900.016.21087623
CGCTACC148500.015.73908425
TCACGCT164750.015.1696588
CGCTATG29250.014.84980729
CGCGTAA38150.014.803651
AAAACGC160250.014.71129221
GCCTACG26500.014.51567910
TCGCTAT30950.014.24941728
CGTCTGA54550.014.14447916
CGACGTC31300.013.9426723
Produced by FastQC (version 0.11.5)