FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41780469
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC923950.2211439991255244No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG771190.18458146077776197No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG702080.1680402390887474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG196100.023.09595944
ACGCTAC325450.022.64816924
TCACGCT330450.022.625528
AACGCTA325900.022.58248523
CGCTACC329750.022.24365825
TTCACGC338100.022.2134067
TCGGCGC205450.022.11057345
AAAACGC341400.021.62970421
GAAAACG345000.021.38515320
AAACGCT348200.021.27837622
ACGCTCA360150.021.11593810
CGTGGTT349900.020.94390541
GTTTTCG354450.020.70612145
TCGTGGT359700.020.42333240
CGCGTAA16900.020.1141551
AGACCGC91300.019.9364384
CACGCTC387500.019.3002879
GCCGGCT197850.019.1951081
CAGACCG97350.018.951683
ATTCGGC245150.018.59419343
Produced by FastQC (version 0.11.5)