FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41780469
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA777920.18619226126925478No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG443040.10603997767473601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA359700.013.26005845
GCACCGA381100.012.44098944
TCAGACG92050.012.0434732
CGTGGCG185650.011.8215551
GTCACTC183300.011.7897368
ACCCGGC51850.011.709885
GGCATAG87900.011.69181335
ATAGGGG87800.011.65361338
TTTCTAT429250.011.5666751
TTCTATA422850.011.4867192
GGTCACT187300.011.4659027
CCGTACG11800.011.2436524
GGGAGGT211900.011.1745943
AGCATAG101800.011.13647945
TGCCCGT191750.011.11504344
GCGTAGG100450.011.05926936
AGGGATC160800.011.0196752
TCTATAG433600.011.0101953
GGCGTAG101500.010.96714935
GCGTCGT19500.010.95581211
Produced by FastQC (version 0.11.5)