FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45024544
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2475270.5497601485980624No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG555250.12332162653329704No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG464410.10314596412125795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA571800.023.80161523
ACGCTAC573250.023.7219724
AAAACGC587800.023.21830421
CGCTACC586800.023.1435925
GAAAACG589700.022.8364620
AAACGCT597650.022.81724522
GCCGGCT567850.022.4763361
GTTTTCG617150.021.88453945
CGTGGTT624300.021.64157941
TCACGCT642750.021.4601828
ACGCTCA658650.021.44466210
TTCACGC644750.021.4319617
TCGTGGT643500.020.98266440
CCGGCTT641450.020.8982812
TACCTCT678650.020.196328
ACCGCGT115850.020.1770946
CACGCTC687400.020.1708939
AGACCGC110950.020.0723274
AGAAAAC677000.020.00701919
CTACCTC689000.019.87971927
Produced by FastQC (version 0.11.5)