FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44631452
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG946480.21206569752648874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT244050.021.9773961
AACGCTA256650.021.4937823
ACGCTAC259000.021.29892724
CGCTACC265000.020.7827725
TTCACGC275750.020.4998477
CCGGCTT276400.020.2944472
TCACGCT280300.020.1189678
AAAACGC276700.020.03340521
ACGCTCA287750.019.91924910
GAAAACG277600.019.67825520
AAACGCT289200.019.15231122
CGGCTTC330550.018.3428673
CACGCTC313800.018.1287489
GTTTTCG319450.016.95139945
CGTGGTT329300.016.6835641
CGCTCAG347450.016.5294311
TCGTGGT340350.016.05683140
CTACCTC352350.015.96264627
GCTACCT358750.015.77947226
TACCTCT356650.015.70673128
Produced by FastQC (version 0.11.5)