FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44639804
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT216700.013.6488483
ATAGGGG141750.012.97509338
GGCATAG145100.012.4278935
CGTGGCG70950.012.2999991
GGAGGTC241300.012.1167184
AGCTTAG155450.011.51476845
CGTAGGG80850.011.51359137
GGCGTAG81450.011.51159435
GCGTAGG81650.011.51093436
GTTGTCG17200.011.50287126
CACCGAA164950.011.31744745
GAGCTTA162450.010.94770144
CATAGGG165950.010.93419337
TGGCATA167900.010.79387134
TGGCGTA88400.010.63204834
TAGGGGA265650.010.3924939
GTGGCGA84200.010.2848572
AGCATAG95300.010.24499145
GCATAGG181650.010.0015336
CGTAACG13800.09.988934542
Produced by FastQC (version 0.11.5)