FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43516930
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG978650.22488948554045518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT230750.023.2178631
CCGGCTT278000.020.168572
TCACGCT281600.019.8945738
TTCACGC282150.019.8478167
ACGCTCA298700.018.9743910
AACGCTA291350.018.79634923
ACGCTAC292250.018.76155924
CGCTACC296750.018.34816225
CACGCTC307650.018.2465849
AAAACGC309900.017.70753721
CGGCTTC330850.017.5044823
GAAAACG313750.017.46875620
AAACGCT321800.017.13662122
AGACCGC33850.016.6170234
CGTGGTT334450.016.36736741
CCCAGAT177200.015.9555931
TCGTGGT342850.015.94010840
CGCTCAG362950.015.84580811
GTTTTCG341000.015.7824645
ACCGCGT38550.015.0578596
Produced by FastQC (version 0.11.5)