FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43516930
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA825150.18961585755245144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA286150.015.220757545
GCACCGA296900.014.6695544
ATAGGGG141850.013.66524138
GGGAGGT233450.013.5927213
GGAGGTC234750.013.0403774
GGCATAG149200.012.84226935
TTCTATA371500.012.3961432
GTTGTCG18150.012.37118826
ACTCTCC242150.012.26289111
TTTCTAT385500.012.2191181
AGCACCG359850.012.09710743
AGCTTAG160300.012.03362145
TCTATAG382300.011.7529553
CGTGGCG77150.011.7057881
TGGCATA168250.011.52140834
CGTGTAG381850.011.49704336
TAGAGCA398600.011.49152540
GAGCTTA168450.011.45132744
CTATAGT388800.011.4471674
ATAGTGT396400.011.3805476
Produced by FastQC (version 0.11.5)