FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45167649
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG137700.014.09366438
GGCATAG140150.013.60665135
GGGAGGT247400.013.1297373
GGAGGTC250550.012.4338854
CACCGAA201800.012.33105645
GTCACTC242350.012.2943338
GTTGTCG23700.012.049226
TGGCATA163250.011.95693534
GGTCACT252400.011.8315567
ACTCTCC253650.011.720211
GAGCTTA165750.011.674273544
CATAGGG167500.011.54606737
AGCTTAG170150.011.49279745
CGTAGGG87900.011.33353337
GCGTAGG88100.011.30775436
GGCGTAG89500.011.28162935
CGTAACG15100.011.2147342
GAGGTCA282050.011.1305475
TGCCCGT121550.010.86684544
TAGGGGA273850.010.7811139
Produced by FastQC (version 0.11.5)