FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42458517
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26300.015.38762626
CACCGAA203000.012.68059445
GGGAGGT182800.012.6360193
GGAGGTC181600.012.4093184
GCGTCGT21900.012.217483511
GGCATAG127250.012.05106535
ATAGGGG133200.011.86695338
CGTGGCG62000.011.8260561
ATATAGC43750.011.66589456
GCACCGA223950.011.53335544
TGTCGTT37950.011.07863528
TCGTGTA24900.010.92617114
TTCTATA263800.010.6920952
GAGCTTA147050.010.52654844
CGTAACG15700.010.5071242
TGGCATA152500.010.27695334
GTCGTTC40000.010.2299629
AGCTTAG153350.010.22792645
CATAGGG152900.010.20588937
GGCGACC72450.010.1509214
Produced by FastQC (version 0.11.5)