FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41187007
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA428000.10391626660320329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA193050.011.86165245
GGGAGGT165200.011.7471493
GCACCGA194150.011.69815744
CGTGGCG77450.011.6422031
ATAGGGG103200.011.39633838
GGCATAG105400.011.22291935
TTCTATA223600.010.8913862
ACTCTCC168950.010.71510311
GGAGGTC183100.010.6971324
TCTATAG219350.010.6924793
GCGTAGG54350.010.63363336
GACGCAA42350.010.55525244
GGCGTAG54800.010.5466235
CGTAGGG55300.010.49162337
GTTGTCG16300.010.48497226
GTCACTC173400.010.4527328
ACTAACC137400.010.3272838
CTATAGT228100.010.2824494
GGTCACT177050.09.9705037
CATAGGG120850.09.9553237
Produced by FastQC (version 0.11.5)