FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41369670
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA965190.23330860507226672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT352000.015.1073271
CACCGAA358500.015.06222545
GCACCGA365750.014.8250944
AGGGATC142550.014.2742322
TTCTATA415250.013.8154322
TCTATAG423600.013.2628133
AGCACCG416100.013.04741543
CAGGGAT188700.012.2320121
CTATAGT458050.012.1823654
GTTGTCG20500.011.8472226
TATAGTG477550.011.8397925
GAGCACC461850.011.82806442
CGTGGCG64650.011.7160371
ATAGGGG108250.011.67579338
CGTAACG17550.011.54277642
GCGTCGT17300.011.52930711
ATAGTGT496500.011.5234916
TAGAGCA496650.011.52067440
GGAGGTC165800.011.5159624
GTAGAGC484400.011.49610139
Produced by FastQC (version 0.11.5)