FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44329118
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1131670.25528818326590663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT256550.028.5065021
CCGGCTT325100.023.6340472
AACGCTA300100.021.77256823
ACGCTAC302000.021.62068724
TTCACGC359950.021.6073477
TCACGCT366800.021.3326348
CGCTACC307550.021.15736625
ACGCTCA374250.020.94437810
GAAAACG365600.020.81264720
CGGCTTC381400.020.7343943
AAAACGC321100.020.45374521
CACGCTC397150.019.742269
AAACGCT335450.019.5921822
AACGTTA57400.019.55994823
CGTGGTT403050.018.75600241
ACGTTAC61450.018.49049624
TCGTGGT423200.017.9427140
CGCTCAG437650.017.94130511
GTTTTCG417050.017.75414345
TACCTCT461200.016.95676828
Produced by FastQC (version 0.11.5)