FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45106042
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG716040.1587459170104085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT275500.021.2490161
GAAAACG226800.020.06223520
CCGGCTT313400.019.1133082
GACCGCG49100.018.7611835
AACGCTA322250.018.3237323
ACGCTAC324350.018.20521424
TTCACGC337950.018.1562827
TCACGCT335150.018.1270228
AGACCGC49050.018.0018314
CGCTACC288000.017.85819825
ACCGCGT52250.017.808766
AAAACGC338000.017.5427221
ACGCTCA353100.017.29565810
AAACGCT352550.016.81899822
CACGCTC361650.016.7740149
CGGCTTC380900.016.646233
CAGACCG56700.016.326163
CGTGGTT364800.016.11244641
CGCGTTT70050.015.6874891
TCGTGGT379300.015.479341540
Produced by FastQC (version 0.11.5)