FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43652238
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG520130.1191531119206305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC149250.018.23727824
AACGCTA149500.018.20678123
AGACCGC45550.017.780254
CGCGTAA22200.017.4319521
CGCTACC155800.017.34061625
GCCGGCT181850.017.1359041
AAAACGC182700.016.83135221
GAAAACG186050.016.39528720
CAGACCG51400.016.281943
AAACGCT178050.015.42633222
ACCGCGT53150.015.3635776
TCACGCT206300.015.3420678
GACCGCG52950.015.1679215
CGTGGTT201800.014.88792541
TCGGCGC101400.014.82178945
TTCGGCG101050.014.78470944
GCCTACG26950.014.69071310
TTCACGC217500.014.6761497
CCGGTAT37950.014.5845791
TTCGACG17450.014.5670626
Produced by FastQC (version 0.11.5)