FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43652238
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT206150.013.8068257
GTCACTC211050.013.6245978
GGCATAG139850.013.01327535
CGTAGGG80400.012.78642837
ATAGGGG146850.012.7293538
GGGAGGT245850.012.6881593
ACTCTCC227450.012.63239311
GGCGTAG81650.012.50835535
GCGTAGG82000.012.48228636
GGAGGTC243700.012.3660264
CACTCTC232350.012.07524610
CACCGAA236750.011.51452545
CATAGGG158900.011.48116837
CGTGGCG110600.011.4707371
TCACTCT253850.011.4601189
TGGCGTA91450.011.41406334
GCACCGA235200.011.36121944
AGCTTAG163300.011.33566745
GAGGTCA267800.011.303295
TGGCATA168100.010.92014734
Produced by FastQC (version 0.11.5)