FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44310725
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA557890.12590405595936424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC210200.013.167588
GGGAGGT230500.012.9992033
ATAGGGG131500.012.89719738
CGTGGCG79900.012.6629421
GGTCACT215950.012.6086087
TCTATAG261850.012.130653
ACTCTCC225400.012.11015711
CACCGAA253450.012.03377545
GGCATAG139850.011.91772135
GCGTAGG90650.011.88540636
CGTAGGG90600.011.84231137
GGCGTAG91950.011.83943835
GCACCGA262950.011.62468744
TCACTCT247200.011.6066539
CGACCCG87150.011.5672856
TTCTATA280050.011.553622
TTTCTAT291150.011.4004241
GGAGGTC260250.011.3142844
GAGGTCA263800.011.179275
CACTCTC249200.011.00779810
Produced by FastQC (version 0.11.5)