FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41132082
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA441890.10743195542593734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT42250.027.46481511
TCGTGTA43500.026.15916814
GGTCGCC44550.025.8469343
GTCGCCG47150.024.61319244
GTCGTGT51400.023.14461313
CGTCGTG49800.023.12034412
AGCGTCG53400.022.2353110
TGGTCGC53100.021.89789842
ATCTCGG59150.020.37807735
AGAGCGT66000.017.8196568
TCTCGGT68050.017.34897436
TCGCCGT64600.017.3405145
CTCGGTG72950.016.86182637
GATCTCG79200.015.4747834
AGTGTAG80000.014.6447328
TGTAGGG80650.014.4441617
GTAGGGA75750.014.36910118
GTAGATC79900.014.29765631
AACTCTA158000.013.70800445
CACCGAA171250.013.68924345
Produced by FastQC (version 0.11.5)