FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45124982
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC41550.017.8689144
AACGCTA143000.016.40753623
ACGCTAC145550.016.18195524
CGCTACC148750.015.712872525
TCGGCGC93100.015.56351945
TTCGGCG94550.015.46871544
AAAACGC156550.015.10218921
CGCGTGT10750.015.0718431
CCGGTAT40550.014.761591
CCGTACG97000.014.4965124
GAAAACG162900.014.48587420
CGTACGC98950.014.4603955
AAACGCT169200.013.9333522
ACCGCGT55750.013.7997896
CCCGTAT23650.013.7968261
CAGACCG55700.013.7738353
TTCGACG10950.013.764236
TACGCCA109650.013.7043397
CGTGGTT169750.013.68168941
GTTCCTA63300.013.64639115
Produced by FastQC (version 0.11.5)