FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43258778
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA102250.020.85997223
ACGCTAC103550.020.61986724
CGCTACC108150.019.63906125
AAAACGC117500.018.19065921
GCCGGCT140700.017.0858541
GAAAACG126950.016.89182720
AAACGCT132750.016.23662422
TCACGCT139600.016.1971238
CCCGTAT33500.015.7228261
TTCACGC145450.015.6848887
GTCCGTT30600.015.594591
CCGGCTT165300.015.3261392
ACGCTCA152850.014.55765910
CGTGGTT151650.014.19837941
GTTTTCG155250.013.72413845
CTCGGTT80400.013.6622841
CGTTACG18000.013.49938421
TCGTGGT161050.013.49539540
CCCGGAT96050.013.4281681
TCGGCGC83150.013.25868945
Produced by FastQC (version 0.11.5)