FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43258778
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG147750.014.96948838
GGCATAG146200.014.91326535
AGCTTAG155750.014.29951945
GTCACTC233350.013.4089228
GGTCACT233700.013.16776857
GGGAGGT254450.013.1615433
GAGCTTA171650.012.94857644
CATAGGG171850.012.88326737
ACTCTCC249750.012.54663511
TGGCATA175050.012.53271434
GGAGGTC257000.012.3225954
GCATAGG181750.012.20649936
CACTCTC263350.011.72799810
CACCGAA124850.011.43918745
TCACTCT283850.011.2531919
TAGGGGA277500.011.07213339
GCACCGA137250.010.45517444
TCTGTCC301050.010.41560424
GAGGTCA311050.010.4063835
TCTCCCC303100.010.35308913
Produced by FastQC (version 0.11.5)