FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44223568
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA564820.12771922880578068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG135550.014.30457638
GTCACTC211050.013.2942938
GGGAGGT222900.013.0462453
GGCATAG148100.012.98673335
GGTCACT222900.012.6181997
ACTCTCC233100.012.44232811
GGAGGTC234950.012.3291934
AGCTTAG171250.011.51834645
CATAGGG170050.011.42914537
GAGCTTA170150.011.40800644
TGGCATA174550.011.17368734
CACCGAA255000.011.11351645
CACTCTC258800.010.90217110
GTTGTCG17550.010.89756426
GAGGTCA267700.010.8794315
TCACTCT266200.010.7003279
GCGTAGG78650.010.69690936
TAGGGGA268500.010.68152239
CGTAGGG79050.010.67102137
GCACCGA265700.010.61541444
Produced by FastQC (version 0.11.5)