FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42877920
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG101450.012.88192838
GGCATAG107550.011.85793835
AGCTTAG111900.011.51770945
CGTGGCG89200.011.3168011
GGGAGGT182350.011.1597853
CATAGGG121250.010.70406737
TGGCATA122900.010.54188434
CACCGAA146250.010.47352445
GAGCTTA123850.010.33361544
GGAGGTC196350.010.0430684
GTGGCGA99850.010.0414432
CGACCCG104200.09.7610436
AACTAAC124000.09.75922637
TGGCGAC105850.09.7189313
ACTAACC129100.09.60024238
GCGACCC109700.09.5216825
GCATAGG136450.09.4951936
GCACCGA163900.09.37298144
GTTGTCG17650.09.30493626
CGTGTAG162250.09.2330336
Produced by FastQC (version 0.11.5)