FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45827291
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG938360.20476008498953166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT220650.023.0289751
AACGCTA226850.022.4754723
ACGCTAC227950.022.36701224
CGCTACC229450.022.11292325
GAAAACG235500.021.1141420
CCGGCTT253350.021.0832712
AAAACGC244300.020.9253421
TTCACGC265100.020.454147
AAACGCT250750.020.3870822
TCACGCT271400.020.1116968
ACGCTCA272450.019.8691110
CACGCTC289400.018.7986019
GTTTTCG278250.018.2580645
CGGCTTC322250.017.4272693
TCGTTAC21350.017.2826944
CGCTCAG321050.017.10019311
CTACCTC312400.016.5583327
TACCTCT316450.016.35288228
TTCCTCG319050.016.2197636
CGTGGTT321100.015.947865541
Produced by FastQC (version 0.11.5)