FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45397644
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1121000.24692911376634433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT271200.021.6055871
TTCGACG21350.019.6000826
CCGGCTT324600.018.9850062
AACGCTA323550.018.4769323
ACGCTAC324550.018.46852724
CGCTACC327900.018.24553525
TTCACGC344650.017.82087
GTTTTCG334850.017.69131745
CGTGGTT339850.017.57668141
AAAACGC343400.017.48095721
TCACGCT352350.017.4313568
GAAAACG344550.017.30958620
TCGACGA24050.017.3061127
AAACGCT351650.017.09003422
ACGCTCA357650.017.06000310
CGGCTTC392650.016.4331633
CGGTATA30500.016.23363920
CACGCTC386450.015.9632329
CCCGTAT24000.015.8510141
CGACGAG24350.015.7993788
Produced by FastQC (version 0.11.5)