FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49400529
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1344720.27220761138003197No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG941530.19059107646397874No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCC756530.15314208477403146No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTC629000.1273265717458208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT348150.024.3669011
GACCGCG52700.022.5846945
TTCACGC629900.021.8855427
TCACGCT635350.021.6128168
ACCGCGT55250.021.5429126
CCGGCTT428600.021.1244752
ACGCTCA668250.020.72722210
CACGCTC672900.020.5037389
GAAAACG596650.020.33848620
AGACCGC60450.019.9497574
AACGCTA691000.019.51352923
ACGCTAC692350.019.4754824
CGCTACC698250.019.27867525
CGCTCAG720500.019.20848811
AAAACGC706750.019.06915521
CGGCTTC492500.018.9132963
AAACGCT719650.018.7335522
CGTGGTT765300.017.49557741
CTTCACG811450.017.4215326
GCTCAGG785100.017.37599812
Produced by FastQC (version 0.11.5)