FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45763218
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG930460.20332049201609906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT270900.019.5176541
CCGGCTT292700.018.96322
AACGCTA279050.018.83466323
ACGCTAC279900.018.79356424
CGCTACC283400.018.5379525
TTCACGC292100.018.4933997
TCACGCT298300.018.0713128
ACGCTCA305000.017.95490310
AAAACGC297700.017.78293821
GAAAACG301950.017.47551320
AAACGCT311950.017.04280522
CGTGGTT303400.016.61105241
CACGCTC328750.016.5140259
CGGCTTC363600.016.1007373
GTTTTCG325000.016.04699945
GTTCCTA80900.015.6329615
TCGTGGT323250.015.59798440
CCCGTAT32200.015.5185341
CGCTCAG371500.014.79578111
CCTCGTG361250.014.47414838
Produced by FastQC (version 0.11.5)