FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45763218
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA800410.17490247298605618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA304700.012.91844545
GGGAGGT233950.012.8342033
GCACCGA316950.012.41231144
ATAGGGG146450.012.27828838
GGCATAG149300.011.83337135
GGAGGTC261100.011.758394
TTCTATA368800.011.3493722
TCTATAG357950.011.2471213
CGTGGCG80650.011.1293651
GTCACTC265350.010.95305258
TTTCTAT396800.010.7376741
AGCTTAG164850.010.6550645
GGTCACT270750.010.6018117
TCACTCT274700.010.588619
ACTCTCC275450.010.54350411
GTTGTCG21700.010.46182326
AGCACCG387700.010.24064743
CTATAGT392100.010.2276664
CGTAGGG105350.010.19836537
ATAGTGT404250.010.1922276
Produced by FastQC (version 0.11.5)