FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45445946
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG525060.11553505784652388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA238300.019.7612123
ACGCTAC240100.019.61306224
CGCTACC246700.019.13395125
CCGGCTT284350.018.3970472
AAAACGC257450.018.3525221
ACGCTCA273000.018.18152610
GCCGGCT279950.017.776541
GAAAACG266800.017.66033220
AAACGCT270900.017.47451222
ACCGCGT42250.017.4676366
TCACGCT285800.017.3042358
TTCACGC286950.017.250577
GACCGCG44950.016.567875
AGACCGC45650.016.2152984
CGGCTTC344550.015.8224713
CGTGGTT305000.015.73476841
CAGACCG48800.015.6758913
CACGCTC322850.015.4856969
TCGTGGT321550.014.93893440
CTACCTC332000.014.60419327
Produced by FastQC (version 0.11.5)