FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41047495
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1033970.25189600485973623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT236500.023.6316591
CCGGCTT273300.020.8542182
TTCACGC288450.019.9220437
TCACGCT294500.019.3882228
ACGCTCA294450.019.36887710
ACGCTAC290400.019.12234124
AACGCTA291400.019.03267323
CGCTACC293850.018.7985425
CGGCTTC317300.018.5438523
AAAACGC307100.018.04403721
CACGCTC319850.017.8306669
TTCGACG12150.017.7778196
GAAAACG312350.017.74064420
GACCGCG29050.017.7368535
AAACGCT317450.017.51256422
CGCTCAG354150.016.2689811
GTTTTCG334800.016.2302745
CGTGGTT341550.016.16002741
ACCGCGT32250.016.0465496
AGACCGC31900.016.0111454
Produced by FastQC (version 0.11.5)